2014-06-18 · In plants, brassinosteroids (BR) counter the suppressive effect of abscisic acid (ABA) on early seedling development. Ryu et al.show that ABA regulator genes are suppressed by a complex containing

Although the mechanisms by which the expression of ABI5 is regulated by ABI3 are unknown, a key observation of our work is that ABI5 can complement abi3‐1, whereas ABI3 cannot complement abi5‐4. This strongly suggests that ABI5 acts downstream of ABI3 to arrest growth of germinated embryos by ABA.

2018-02-20 · ABI5 has been implicated as the final inhibitor of seed germination, possibly acting downstream of the GA repressor RGL2 [ 16, 17 ]. The COP9 signalosome (CSN) is a conserved heteromeric protein complex known to regulate the CULLIN-RING family of ubiquitin E3 ligases (CRLs), including the SCF sub-family of E3s [ 21 ]. Abscisic acid‐insensitive 5 (ABI5) is an essential and conserved plant basic leucine zipper transcription factor whose level controls seed germination and postgerminative development. It has been demonstrated that activity of ABI5 is transcriptionally and post‐translationally regulated. Transgenic plants of ABI5-5, ABI5 S42A, ABI5 S145A, and ABI5 S42AS145AT201A were generated by Agrobacterium tumefaciens GV3101-mediated floral infiltration (Clough and Bent, 1998). To overexpress FLC in the abi5-4 mutant background, plasmid p35S::GFP-FLC was transformed into abi5-4 plants.

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ABI5 functions in the core ABA signaling, which is composed of PYR/PYL/RCAR receptors, PP2C phosp … 2019-03-01 · In turn, ABA promotes the binding of HY5 to ABI5, and transgenic plants overexpressing ABI5 display shorter hypocotyls, indicating that ABI5 enhances light responses. HY5 is a highly conserved central regulator of photo-morphogenesis [ 57 , 58 ] that acts downstream of phytochrome-A and UV RESISTANCE LOCUS8 photoreceptors [ 59 , 60 ]. Our results show that ABI5 plays a key role in seedling growth under photorespiratory conditions by regulating photorespiratory enzyme expression, and ABA regulates plant growth under low CO 2 conditions through modulating ABI5 expression.

24 Mar 2020 Growth arrest phenotype of ppc2 mutant seedlings under low CO2 conditions. (A) Genotyping and (B) expression analysis of PPC1, PPC2, and

The abi5 mutant (SALK_013163; Zheng et al., 2012) was obtained from the ABRC, and the T‐DNA insertion is approximately 1 kb upstream of the ABI5 transcription start site. qRT‐PCR analyses showed that the transcription levels of ABI5 and RAV1 were markedly decreased in the RAV1‐U abi5 double mutant (Figure S6). Abi5 LLC is a Florida Domestic Limited-Liability Company filed On February 22, 2021. The company's filing status is listed as Active and its File Number is L21000090327.

Rabbit polyclonal ABI5 antibody. Tested Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Sumoylation of ABI5 by the ArabidopsisSUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling Kenji Miuraa,b,1, Jiyoung Leec,2, Jing Bo Jina,3, Chan Yul Yooa, Tomoko Miuraa, and Paul M. Hasegawaa,1 ABI5 Our initial mapping of ABI5 localized it to the lower arm of chromosome 2, near the phenotypic marker pyrimidine-requiring (py) (Finkelstein, 1994). To generate fine-mapping populations with closely linked recombinations, we out-crossed abi5-1 and abi5-3 (in the Wassilewskija and Colum-bia [Col] backgrounds, respectively) to lines carrying ABI5 expression is greatly reduced in abi3‐1 mutants, which has low AtEm1 or AtEm6 expression. Cross complementation experiments showed that 35S‐ABI5 could complement abi3‐1, whereas 35S‐ABI3 cannot complement abi5‐4.

Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. 2015-09-01 · ABI5 is the best-studied key molecule in the ABA signaling pathway with respect to PTMs; therefore, this review could serve as a model to guide post-translational studies of important regulators in other plant hormone signaling pathways. ABI5 is the best-studied key molecule in the ABA signaling pathway with respect to PTMs; therefore, this review could serve as a model to guide post-translational studies of important regulators in other plant hormone signaling pathways. Keywords: (de-)phosphorylation; ABA signaling; ABI5; sumoylation; ubiquitination. As a crucial regulator of ABA signaling, ABSCISIC ACID-INSENSITIVE5 (ABI5) is involved in many aspects of plant growth and development, yet its regulation of anthocyanin biosynthesis has not been elucidated. In this study, we found that MdABI5, the apple homolog of Arabidopsis ABI5, positively regulated ABA-induced anthocyanin biosynthesis. ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that plays a key role in the regulation of seed germination and early seedling growth in the presence of ABA and abiotic stresses.
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during de-etiolation (Fig.2). Although formation of the apical hook and its light-regulated opening is known to be tightly Although the mechanisms by which the expression of ABI5 is regulated by ABI3 are unknown, a key observation of our work is that ABI5 can complement abi3‐1, whereas ABI3 cannot complement abi5‐4.

Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters.
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The preservation of our genetic resources and production of high-quality seeds depends on their ability to remain viable and vigorous during storage. In a quantitative trait locus analysis on seed longevity in Medicago truncatula , we identified the bZIP transcription factor ABSCISIC ACID INSENSITIVE5 (ABI5).

ABI5 functions in the core ABA signaling, which is composed of PYR/PYL/RCAR receptors, PP2C phosp … 2019-03-01 · In turn, ABA promotes the binding of HY5 to ABI5, and transgenic plants overexpressing ABI5 display shorter hypocotyls, indicating that ABI5 enhances light responses. HY5 is a highly conserved central regulator of photo-morphogenesis [ 57 , 58 ] that acts downstream of phytochrome-A and UV RESISTANCE LOCUS8 photoreceptors [ 59 , 60 ]. Our results show that ABI5 plays a key role in seedling growth under photorespiratory conditions by regulating photorespiratory enzyme expression, and ABA regulates plant growth under low CO 2 conditions through modulating ABI5 expression. abi5-1 seedlings grown at low CO 2 showed lower F v /F m values (Fig. 6C, D). Genetic and physiological evidence has demonstrated this key role of ABI5 in this process. Overexpression of ABI5 resulted in enhanced sensitivity to ABA treatment, and the abi5 mutant was insensitive to ABA treatment during both seed germination and vegetative growth (Finkelstein & Lynch, 2000; Lopez‐Molina et al., 2001; Tezuka et al., 2013). Beyond germination, ABA further inhibits postgermination seedling development, which is often mediated by the transcription factor ABSCISIC ACID INSENSTIVE5 (ABI5) (Chen et al., 2020).